Enke et al. 2007

Eukaryot. Cell, 6:555-562

The Aspergillus nidulans Dis1/XMAP215 protein AlpA localizes to microtubule plus ends and contributes to growth directionality. Eukaryot. Cell, 6:555-562.

Enke, C., Zekert, N., Veith, D., Schaaf, C., Konzack, S. & Fischer, R.



The dynamics of cytoplasmic microtubules (MTs) is largely controlled by a protein complex at the MT plus end. In Schizosaccharomyces pombe and in filamentous fungi, MT plus end-associated proteins also determine growth directionality. We have characterized the Dis1/XMAP215 family protein AlpA from Aspergillus nidulans and show that it determines MT dynamics as well as hyphal morphology. Green fluorescent protein-tagged AlpA localized to MT-organizing centers (centrosomes) and to MT plus ends. The latter accumulation occurred independently of conventional kinesin or the Kip2-familiy kinesin KipA. alpA deletion strains were viable and only slightly temperature sensitive. Mitosis, nuclear migration, and nuclear positioning were not affected, but hyphae grew in curves rather than straight, which appeared to be an effect of reduced MT growth and dynamics.