The DNA segment was sequenced
that
links the nir-nor and nos gene clusters for
denitrification
of Pseudomonas stutzeri ATCC 14405. Of 10 predicted gene
products,
four are putative membrane proteins. Sequence similarity was detected
with
the subunit III of cytochrome-c oxidase (ORF175), PQQ3 of the
biosynthetic
pathway for pyrrolo-quinoline quinone (ORF393),
S-adenosylmethionine-dependent
uroporphyrinogen-III C-methyltransferase (ORF278), the cytochrome cd1
nitrite reductase and the NirF protein involved in the biosynthesis of
heme d1 (ORF507), LysR type transcriptional regulators
(ORF286),
short-chain alcohol dehydrogenases (ORF247), and a hypothetical
protein,
YBEC, of Escherichia coli (ORF57). The current data together
with
previous work establish a contiguous DNA sequence of 29.2 kb comprising
the supercluster of nos-nir-nor genes for denitrification in
this
bacterium.