Pomowski A, Zumft WG, Kroneck PM, Einsle O. (2010)
Crystallization of purple nitrous oxide reductase from Pseudomonas
stutzeri.
Acta Crystallogr Sect F Struct Biol Cryst Commun.
2010; 66(Pt 11):1541-1543.
Nitrous oxide reductase (N2OR) from Pseudomonas stutzeri catalyzes the final
step in denitrification: the two-electron reduction
of nitrous oxide to molecular dinitrogen. Crystals of the enzyme
were grown under strict exclusion of dioxygen by
sitting-drop vapour diffusion using 2R,3R-butanediol
as a cryoprotectant. N2OR crystallized in
either space group P1 or P6(5). Interestingly, the key
determinant for the resulting space group was the crystallization temperature. Crystals belonging to
space group P1 contained four 130 kDa dimers in the asymmetric unit, while crystals belonging to
space group P6(5) contained a single dimer in the asymmetric unit. Diffraction
data were collected to resolutions better than 2 Å.