The multicopper proteins, nitrous-oxide reductase (N₂OR) and cytochrome c oxidase (COX), were investigated by EPR spectroscopy at microwave frequencies 2.4 - 35 GHz. Our results support a Cu-Cu interaction in COX and N₂OR. At least 10 lines in the 2.7-GHz, 12 lines in the 4.6-GHz and 14 lines in the 9.2 GHz spectra were resolved for N₂OR. Eight copper lines at 2.7 GHz, about nine lines at 4.6 GHz and about six lines at 9.2 GHz were resolved for COX. Simulations of the EPR spectra were consistent with most of the resonances of the multiline spectra, including regions in the center of the spectra where overlap of the three seven-line patterns is proposed. These simulations indicated that Cu-Cu interaction, in a nuxed-valence [Cu(1.5)...Cu(1.5)], S = 1/2 site is consistent with, if not proof of, the unusual spectral features observed for N₂OR and COX.